genetics
nasser pouladi; mohammadali hosseinpourfeizi
Volume 25, Issue 3 , July and August 2018, , Pages 399-407
Abstract
Background: Thyroid cancer is the most common malignant endocrine tumor, and the incidence was rising worldwide over the last three decades. TP53 is one of the most important tumor suppressor genes in the genome, and its mutations are found in approximately 50% of human cancers. It plays pivotal roles ...
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Background: Thyroid cancer is the most common malignant endocrine tumor, and the incidence was rising worldwide over the last three decades. TP53 is one of the most important tumor suppressor genes in the genome, and its mutations are found in approximately 50% of human cancers. It plays pivotal roles in the regulation of cell cycle arrest and apoptosis. The aim of this study was to analyze TP53 gene mutations among thyroid cancer patients in East- Azerbaijan. Material and Methods: 40 tumor samples have been collected from thyroid cancer patients from Tabriz hospitals between 2007-2009. DNA was extracted by Proteinase K – Iso propanol method and then the mutations in p53 gene from exon5 to exon8, were detected by Polymarase Chain Reactions (PCR) and direct sequencing techniques.Results: Alterations in the p53 gene were detected in 12.5% of the patients, including single nucleotide polymorphisms and mutations, including codon216 (GTG>GTA), codon215 (AGT>ATT) and codon273 (CGT>CAT) mutations (each mutation in one of the cases) and two linked polymorphisms 14181C>T and 14201T>G (in two cases).Conclusion: This result helps us to clarify p53 mutation status among Azerbaijani thyroid cancer patients.